A consideration left out in this discussion is the type of instrument used to do the measurements. PE saturates at rather low laser power. Thus, PE measured on a BD-Facscan type instrument with low (~15 mW) laser power, efficient light collection optics, and (relatively) slow flow is much better that PE measured on a jet-in-air sorter with higher (several hundred mW) laser power, inefficient optics, and fast flow. APC, to my knowledge, does not have this problem. This may influence the final results obtained with the two dyes. Also, in addition to the doubled YAG, you might try the 568 nm line of a krypton laser. This should hit PE right near its excitation peak, and may make it a better choice than APC. Marty Bigos Operations Manager Stanford Shared FACS Facility bigos@stanford.edu (voice) 650-723-6959 (fax) 650-725-8564
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