Interesting comment there Howard. It may allow some people with multi-line Argon lasers to use the 528.7nm line for PE, losing FITC of course. Bob -----Original Message----- From: Howard Shapiro [mailto:hms@shapirolab.com] Sent: Thursday, 11 November 1999 14:32 To: Cytometry Mailing List Subject: Re: PE or APC > >>Who can tell me? What is better to detect a rare molecule: one molecule of >>PE or one molecule of APC? Mario Roederer, in a well thought-out and comprehensive answer to this question, pointed out: > >In a vacuum of background fluorescence, PE >would be the choice, since it carries more fluors than does APC. >However, if you are measuring against a cell, then APC would probably >be the winner, since the background autofluorescence is much lower. > That's true if you use 488 nm excitation for PE; however, if you use 532 nm excitation (doubled YAG laser), there is almost no autofluorescence (from mammalian cells), and the pendulum swings back toward PE. -Howard
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