Greetings, We are running Indo-1 for calcium flux plus PE and PE-Cy5 for surface markers on an EPICS 753. When samples are run that have been incubated with a drug of interest, we get a higher baseline reading for the indo-1 ratio. If we can prove that the drug is causing the higher baseline through increased background fluorescence in one of the indo-1 channels, is it proper to normalize the ratio baseline by either adjusting PMT voltages or sliding the mean ratio histograms so that the baselines match or none of the above? Thanks. ------------------------------------------------ | Justin Fishbaugh | | University of Iowa | | Flow Cytometry Facility | | 48 EMRB | | Iowa City, IA 52242 | | | | justin-fishbaugh@uiowa.edu | | http://www.medicine.uiowa.edu/flowcytometry/ | ------------------------------------------------
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