Hi Joan, When one uses the "live gate" approach it is always necessary to collect also a smaller file (eg 10000 events) from the total cellularity to be able to obtain the frequency of various populations in the total sample. Otherwise one can only get the percentages in the population the "live gate" was set on. Best wishes Anna Anna Porwit Heamatopathology Lab. Department of Pathology Karolinska Hospital, Stockholm anpo@mb.ks.se tel.:+46-851774518 fax.:+46-851775843
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