Richard- I have enclosed excitation and emission spectra of TR as PICT and TIFF files; hopefully you can open one of them. At 514 nm the excitation efficiency of TR is only a few %. Thus it will be very difficult to see much antibody expression with this choice of fluorochrome. We use a dye laser set to 595 nm for TR measurements (this has been done here for over 10 years). At that excitation wavelength TR is a reasonably good fluorochrome. Hopefully your user can be educated to choose a more suitable fluorochrome. >I have an investigator that has designed an experiment that lends itself to >using Texas Red as the fluorochrome of choice. She has acquired an antibody >congugated with Texas Red for an assay that is specific to her needs. >Unfortunately, she failed to inform the operator of our Facstar Plus about >her choice of fluorochrome. Our instrument is equipped with a dual laser >system with one laser limited to a single 488nm line. The second laser has >multi-line capabilities, but can only be tuned to the maximum of 514nm. The >optimal excitation wavelength for Texas Red as I have discovered is 590nm. >I have discussed this with her, but she insists on analysis at 514nm. I >cannot envision getting any suitable results. Does anyone out there have >any experience with Texas Red that could shed any light on this problem? > >Thank You > >Richard J. Jaramillo >LRRI >Albuquerque,NM >Ph(505)845-1217 Fax(505)845-1198 >rjaramil@lrri.org
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