I have an investigator that has designed an experiment that lends itself to using Texas Red as the fluorochrome of choice. She has acquired an antibody congugated with Texas Red for an assay that is specific to her needs. Unfortunately, she failed to inform the operator of our Facstar Plus about her choice of fluorochrome. Our instrument is equipped with a dual laser system with one laser limited to a single 488nm line. The second laser has multi-line capabilities, but can only be tuned to the maximum of 514nm. The optimal excitation wavelength for Texas Red as I have discovered is 590nm. I have discussed this with her, but she insists on analysis at 514nm. I cannot envision getting any suitable results. Does anyone out there have any experience with Texas Red that could shed any light on this problem? Thank You Richard J. Jaramillo LRRI Albuquerque,NM Ph(505)845-1217 Fax(505)845-1198 rjaramil@lrri.org
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