On Thu, 28 Oct 1999, Van Bockstaele, Dirk noted his responses to
his questions on sorters.....
I am also interested in these data as I am currently looking for a
new sorter. Would it be possible to have a look at the data?
Best regards
Paul Robinson
Date sent: Mon, 1 Nov 1999 11:40:03 -0600 (CST)
From: Jeffrey M Scott <jeffreys@bcm.tmc.edu>
To: cyto-inbox
Subject: Re: performance of high speed cell sorters
On Thu, 28 Oct 1999, Van Bockstaele, Dirk wrote:
>
> Dear flowers,
> some weeks ago I posted a message for obtaining first hand information
> on how the high speed sorters are working in the real world (as opposed
> to on paper!). I promised you an overview and believe it or not, this
> is very easy: only moflo users (11) and one coulter user responded and
> all moflo users were very enthousiastic about their machine! the
> coulter user has no experience yet with the high speed altra but is very
> satisfied about the elite-esp. From these results it looks as if only
> cytomation builds a high speed machine that makes customers happy and
> that all BD and Coulter users vanish from earth! Well it certainly gives
> me something to think about. Any late responses still are wellcome and
> to those who responded: thank you very much. Dirk
>
> Prof. Dirk Van Bockstaele, PhD
> Laboratory of Hematology
> Head Flow Cytometry
> Antwerp University Hospital
> Belgium
> phone 32 3 821 3900, fax 32 3 825 1148
>
To Whom It May Concern,
I sort almost daily on a BCoulter Altra High Speed Sorter.
We have had it for about a year. We have had lots of success
with it. It has very stable drop delay and side streams.
I have sorted at 32000 events per second with a
resultant purity of 95%+. If I sort below 30000 events/sec
we usually get a purity of over 98%.
We have an Autoclone that is fairly easy to use
and a gated amp for multi-laser sorts. I have not used the gated
amp very much yet. We are very happy with the Altra.
Jeff Scott
Flow Cytometry Core Facility
Baylor College of Medicine
Houston Texas 77030
J.Paul Robinson, Ph.D.,
Professor of Immunopharmacology
Professor of Biomedical Engineering
Director, Purdue University Cytometry Laboratories
Purdue University, West Lafayette, IN 47907-1515
(Ph) (765) 494-0757 Fax (765) 494-0517
jpr@flowcyt.cyto.purdue.edu
http://www.cyto.purdue.edu
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:54:11 EST