On Thu, 28 Oct 1999, Van Bockstaele, Dirk wrote:
>
> Dear flowers,
> some weeks ago I posted a message for obtaining first hand information on
> how the high speed sorters are working in the real world (as opposed to on
> paper!). I promised you an overview and believe it or not, this is very
> easy: only moflo users (11) and one coulter user responded and all moflo
> users were very enthousiastic about their machine! the coulter user has no
> experience yet with the high speed altra but is very satisfied about the
> elite-esp.
> From these results it looks as if only cytomation builds a high speed
> machine that makes customers happy and that all BD and Coulter users vanish
> from earth!
> Well it certainly gives me something to think about.
> Any late responses still are wellcome and to those who responded: thank you
> very much.
> Dirk
>
> Prof. Dirk Van Bockstaele, PhD
> Laboratory of Hematology
> Head Flow Cytometry
> Antwerp University Hospital
> Belgium
> phone 32 3 821 3900, fax 32 3 825 1148
>
To Whom It May Concern,
I sort almost daily on a BCoulter Altra High Speed Sorter.
We have had it for about a year. We have had lots of success
with it. It has very stable drop delay and side streams.
I have sorted at 32000 events per second with a
resultant purity of 95%+. If I sort below 30000 events/sec
we usually get a purity of over 98%.
We have an Autoclone that is fairly easy to use
and a gated amp for multi-laser sorts. I have not used the gated
amp very much yet. We are very happy with the Altra.
Jeff Scott
Flow Cytometry Core Facility
Baylor College of Medicine
Houston Texas 77030
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