Dear Meral, In my opinion, the best way to obtain dendritic cells is to cultivate them out of BM progenitors that are easy to isolate (CD34+ ). The culture in GM-CSF and TNF-a (and maybe some additional cytokines) results in an abundant amount of DC after 2 weeks. You may want to read relevant literature: group of Caux and Bancherau is a good starting point (and endpoint!) We have a modest contribution in: Generation of DC from BM progenitors using gm-csf, tnf-a, and additional cytokines: antagonistic effects of il-4 and ifn-g and selective involvement of tnf-a receptor-1. Lardon et al. Immunology 1997, 91: 553-559 Off course all this is on human DC but I think it also holds for mice work. Best Regards, Dirk Prof. Dirk Van Bockstaele, PhD Laboratory of Hematology Head Flow Cytometry Antwerp University Hospital Belgium phone 32 3 821 3900, fax 32 3 825 1148 > ---------- > Van: keskinte@immagene.mcg.edu[SMTP:keskinte@immagene.mcg.edu] > Verzonden: woensdag 27 oktober 1999 19:41 > Aan: Cytometry Mailing List > Onderwerp: Dentritic Cell Markers > > > Dear Group, > > I have a user that needs to isolate dentritic cells from mice. We would > like to hear about new dendritic cell markers. Any experience and > suggestions would be appreciated. Thanks in advance. > > -------------------------------------------------------------------------- > ---- > > Meral Keskintepe, PhD. > > Institute of Molecular Medicine and Genetics > Medical College of Georgia > 15th street CA-2022 > Augusta, GA. 30912-2600 > > Tel: (706) 721-8880 > Fax: (706) 721-8732 > > -------------------------------------------------------------------------- > -- > -- > >
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