We have been using a Percoll method described by Budzko et al.
Laboratory Investigation 53(5): 586, 1985. We use this for rhesus and
baboon and are satisfied with the yield, purity, viablity, and
functionality. We have noted that the primate MNC preps obtained
using Percoll generally contain fewer granuloctyes than preps made
using Ficoll. However, the Percoll-purified primate preps generally
contain more granulocytes than Ficoll-purified human MNC.
Gib Otten
Chiron Corp.
______________________________ Reply Separator _________________________________
Subject: RE>Primate Mononuclear cell isolation
Author: Henk Niphuis <niphuis@bprc.nl> at SMTP
Date: 10/26/99 8:45 PM
RE>Primate Mononuclear cell isolation Dear Dan
The density is 1.070-1.080 g/ml. and for cyno's (macaca fascicularis) we dilute
this medium with RPMI to a 90% dilution.
Henk Niphuis
Lab of Viral Pathogenesis
Department of Virology
Biomedical Primate Research Centre
Lange Kleiweg 151, 2288 GJ RIJSWIJK
The Netherlands
Tel: +31 15 284 28 55
Fax: +31 15 284 39 86
Email: niphuis@bprc.nl
--------------------------------------
Date: 26-10-1999 20:18
To: cyto-inbox
From: Dan Smith
Anyone out there have a good Ficoll or Percoll mononuclear cell isolation
protWe have good results with LSMRLymphocyte Separation Medium from ICN
Biomedicals Inc. Aurora, Ohio 44202 (1-800-854-530) for rhesus macaques
ocol for primate mononuclear cells?? Is it similar to human or??
Thanks!
Dan Smith
San Diego
Henk Niphuis
Lab of Viral Pathogenesis
Department of Virology
Biomedical Primate Research Centre
Lange Kleiweg 151, 2288 GJ RIJSWIJK
The Netherlands
Tel: +31 15 284 28 55
Fax: +31 15 284 39 86
Email: niphuis@bprc.nl
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