David et al., It is not obvious to me how dCTP could be specific for detecting apoptosis using standard TUNEL reaction conditions. Are there special ionic components of the TdT buffer which somehow allow select for "specific" incorporation of dCTP only onto the ends of DNA generated as a result of apoptosis? I would be very interested in seeing references to published works describing the development and characterization of the procedure. Ty Lee tylee@itis.com -----Original Message----- From: David.McFarland@mcmail.vanderbilt.edu <David.McFarland@mcmail.vanderbilt.edu> To: cyto-inbox Date: Wednesday, October 27, 1999 2:22 PM Subject: tunel again > >I posted a question about tunel a few days ago. I was asking about the >specificity of dctp (deoxy cytosine triphosphate) for apoptosis. According to >the responses I received, it is specific for strand breaks that occur >enzymatically during apoptosis and no dead cell discriminator is needed as it is >when using AnnexinV. Due to my poorly worded question some people thought I was >asking if PI was specific for apoptosis. I know that this isn't the case. >Thanks for clearing that up for me. > > >David McFarland >Howard Hughes Medical Institute >Flow Cytometry Facility >Vanderbilt University Medical Center
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