RE>Primate Mononuclear cell isolation Dear Dan The density is 1.070-1.080 g/ml. and for cyno's (macaca fascicularis) we dilute this medium with RPMI to a 90% dilution. Henk Niphuis Lab of Viral Pathogenesis Department of Virology Biomedical Primate Research Centre Lange Kleiweg 151, 2288 GJ RIJSWIJK The Netherlands Tel: +31 15 284 28 55 Fax: +31 15 284 39 86 Email: niphuis@bprc.nl -------------------------------------- Date: 26-10-1999 20:18 To: cyto-inbox From: Dan Smith Anyone out there have a good Ficoll or Percoll mononuclear cell isolation protWe have good results with LSM®Lymphocyte Separation Medium from ICN Biomedicals Inc. Aurora, Ohio 44202 (1-800-854-530) for rhesus macaques ocol for primate mononuclear cells?? Is it similar to human or?? Thanks! Dan Smith San Diego Henk Niphuis Lab of Viral Pathogenesis Department of Virology Biomedical Primate Research Centre Lange Kleiweg 151, 2288 GJ RIJSWIJK The Netherlands Tel: +31 15 284 28 55 Fax: +31 15 284 39 86 Email: niphuis@bprc.nl
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