Hello to all, I would like to know if any one out there been having problems recovering plasma cells from bone marrow aspirates when they are submitted to be phenotyped by flow cytometry.It seems that I always recover 20-30% less than what the pathologists are estimating with there diff.This is also causing me a problem when i try to do CD38/PI staining to estimate the S-phase of the plasma cells specially when you end up with less than 10% by flow.Any ideas or suggestions to how I can minimize my loss or enrich the recovery of plasma cells to match the diff are welcomed. Faisal Rawas ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
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