Dear Flowers
Many thanks for your responses to my request for lymph node normal ranges -
here's the info for anyone else who might be interested:
There are normal ranges for lymph nodes in Bryan CF et al. Annals NY Acad
Sci 677:404-6, 1993.
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Long time ago I did the effort of collecting a number of normal lymph nodes
and looking at lymphocyte subsets. I never published this but as a rule of
thumb we consider a normal lymphocyte subset distribution within a lymph
node to be T (50%) with large excess of CD4 (relative to CD8), B (50%) with
kappa/lambda ratio = ratio in PB.
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Maiese RL, Segal GH, Iturraspe J, Braylan RC: The cell surface antigen and
DNA content distribution of lymph nodes with reactive hyperplasia. Mod
Pathol 1995; 8(5): 536-543.
TABLE 2. QUANTITATIVE DISTRIBUTION OF CELL SURFACE ANTIGENS
Antigen(s) No. of Cases %Cells Average Fluorescence Intensity (FI)
CD45 57 99 (4) ++
CD2 24 62 (17) +
CD3 46 56 (16) ++
CD5 57 64 (15) ++
CD7 44 54 (14) +
CD4 54 42 (15) ++
CD8 55 15 (10) ++
CD3+CD7+ 34 50 (16) NA
CD4+CD8+ 31 3 (3) NA
CD19 29 36 (16) +
CD20 60 43 (18) ++
CD20+CD5+ 36 * NA
IgG 23 * ++
IgM 22 31 (14) ++
IgA 20 * +
IgD 21 24 (10) ++
Í(CD19) 47 55 (6) ++
Î(CD19) 47 41 (5) ++
Í(CD20) 56 55 (7) ++
Î(CD20) 56 41 (5) ++
CD10 35 4 (6) "
CD14 21 1 (1) -
CD16 11 1 (1) -
CD56 11 4 (5) "
CD13 12 3 (5) "
CD33 15 4 (4) "
CD34 20 0 (1) -
CD71 16 * +
HLA-DR 24 * +++
CD11c 35 * ++
CD23 10 * ++
%Cells fraction of all cells, expressed as mean
(S.D). (The values for Í and Î are fractions of CD19+ or CD20+ cells, as
designated.)
* marker with broad immunofluorescence
expression (Fractions of positive cells in these populations could not be
accurately calculated; see text for further discussion.)
FI fluorescence intensity of surface markers
[(-): = negative control; ("): > negative control and # 10; (+): > 10 and #
102 ; (++): > 102 and # 103; (+++): > 103 and #104]
NA not applicable.
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I can not suppress a need to throw in my two cents. I believe citing normal
ranges for lymph nodes to be potentially misleading, as the range of subset
distribution varies significantly based upon anatomic site (eg. axillary and
inquinal nodes vary from cervical nodes adn vary from mesenteric nodes as to
the distribution of T cells, B cells, and "normal" state of activations of
various subsets). Lymph nodes are not the relatively homogeneous sample as
blood and bone marrow.
###
That's it folks!
Beth Rees
Royal Hobart Hospital
Hobart Tasmania
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