Dear Jeff We always (for sterile sorting) use bags of sterile saline from Baxter, but we fill the tank on the bench in the open ! Very, very rarely have contamination problems. Fran Gibson Dept of Haematology St. George's Hospital Medical School On Fri, 8 Oct 1999 11:20:54 +0200 Van Bockstaele, Dirk wrote: > From: Van Bockstaele, Dirk <Dirk.Van.Bockstaele@uza.uia.ac.be> > Date: Fri, 8 Oct 1999 11:20:54 +0200 > Subject: RE: sheath tank filling > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > > > Dear Jeff, > we are allways filtering and filling sheath fluid tanks in the laminar flow > cabinet. Actually we have trown out all in-line filtering devices because > in our experience they give nothing but trouble (contamination build-up, > partial cloughing and pressure drop, shedding of fibre material in the > sheath line etc...) Off course this was our experience when we started > sorting (i.e. a decade ago), maybe by now there are some better materials > available. > I hope this satisfies your curiousity. > best regards, > Dirk > > > Prof. Dirk Van Bockstaele, PhD > Laboratory of Hematology > Head Flow Cytometry > Antwerp University Hospital > Belgium > phone 32 3 821 3900, fax 32 3 825 1148 > > > > ---------- > > Van: Jeff_Carrell@hgsi.com[SMTP:Jeff_Carrell@hgsi.com] > > Antwoord naar: Jeff_Carrell@hgsi.com > > Verzonden: woensdag 6 oktober 1999 21:41 > > Aan: Cytometry Mailing List > > Onderwerp: sheath tank filling > > > > > > Hello All, > > > > Does anyone fill their cell sorter sheath fluid tank "out in the open", or > > does > > everyone fill the tank in the laminar flow cabinet? > > > > Just curious, > > > > Jeff Carrell > > Human Genome Sciences > >
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