mouse CD45 expression

From: Gillis Otten (Gillis_Otten@cc.chiron.com)
Date: Fri Oct 08 1999 - 19:07:22 EST 

     I would like to use the CD45 mAb, 30F11, to distinguish between 
     leukocytes and non-hematopoietic cells in single cell suspensions 
     prepared from mouse tissue, e.g., lung.  Initial staining and flow 
     cytometry indicates two populations:  CD45+ (quite bright) and CD45-.  
     My question is:  has anyone else done this?  Is CD45 a valid marker 
     for this purpose?  Any caveats?
     
     Gib Otten
     Chiron Corp
     Emeryville, CA

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