Dear Jeff, we are allways filtering and filling sheath fluid tanks in the laminar flow cabinet. Actually we have trown out all in-line filtering devices because in our experience they give nothing but trouble (contamination build-up, partial cloughing and pressure drop, shedding of fibre material in the sheath line etc...) Off course this was our experience when we started sorting (i.e. a decade ago), maybe by now there are some better materials available. I hope this satisfies your curiousity. best regards, Dirk Prof. Dirk Van Bockstaele, PhD Laboratory of Hematology Head Flow Cytometry Antwerp University Hospital Belgium phone 32 3 821 3900, fax 32 3 825 1148 > ---------- > Van: Jeff_Carrell@hgsi.com[SMTP:Jeff_Carrell@hgsi.com] > Antwoord naar: Jeff_Carrell@hgsi.com > Verzonden: woensdag 6 oktober 1999 21:41 > Aan: Cytometry Mailing List > Onderwerp: sheath tank filling > > > Hello All, > > Does anyone fill their cell sorter sheath fluid tank "out in the open", or > does > everyone fill the tank in the laminar flow cabinet? > > Just curious, > > Jeff Carrell > Human Genome Sciences >
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