Jeff, We used to fill in a hood. Later, when I bought a new sorter, space was a premium. So, the hood was expendable. Since then (~3 years) we do it "in the open." No problems. (funny story . . . we had an old 6' containment hood for a while . . . the two times we filled our tanks in the hood, we got contamination . . .) ((how sad is it that I think that's funny?)) Note that "in the open" is relative, though. We take precautions to maintain as clean a fill area as possible, rinse tanks and filtering components with bleach and/or ethanol before filling, cover tanks as they fill, and filter all sheath fluid through 0.2 micron cartridges before use. Keep it clean first . . . easier than decontaminating later. MAK. Jeff_Carrell@hgsi.com wrote: > Hello All, > > Does anyone fill their cell sorter sheath fluid tank "out in the open", or does > everyone fill the tank in the laminar flow cabinet? > > Just curious, > > Jeff Carrell > Human Genome Sciences -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry <http://www.cancer.med.umich.edu/flow_cytometry> phone: 734-647-3216 fax: 734-936-7376 kukuru@umich.edu
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