Paul, While I'm no expert on imaging or QT, I would suggest converting each to a jpeg, since this format presents photos so well, and produces quite compact files. If you have any control over jpeg quality you might want to do a trial run at different settings, because jpegs can often be compressed at 50% quality and still look good enough for the web. I would think the same would be true for movies. (I'm surprised it lets you produce QT's from tiffs, because they're huge! Streaming tiffs would take a LOT of computing power. I'd go for a Linux beowulf cluster ;-) A general caveat for working w/ jpegs--image quality is degraded each time the file is saved, so keep copies of each orginal tiff and work from them! Good luck, any chance we might get to see these videos? Steve ------------------------------------------------------------------ Steve G. Hilliard (flowman@uga.edu) UGA Cell Analysis Facility, Athens GA 30602 ------------------------------------------------------------------ On Mon, 4 Oct 1999, Paul McFadden wrote: > > Dear Confocal Users, > > We have been generating large amounts of Bio-Rad PIC (sometimes totalling > 100MB) files by doing time lapse imaging on living cells. We can export > TIFFS or more compressed (JPEG) images for compiling in Quicktime or the > like for seminars, etc. > > Does anyone have good experince with what file convention to use as it > seems straight tiffs will crash the movie after a few seconds. Because > JPEG is good enough for meetings or seminars we thought the compressed > images would stream faster than the TIFFs. Our goal is to be able to show > these time lapse of cells undergoing apoptosis with 3-color staining. The > file size is huge, can we stream these in a movie format somehow? > > Any replies would be most appreciated... > > Paul R. McFadden > Imaging Facility Supervisor > La Jolla Institute for Allergy and Immunology > 10355 Science Center Drive > San Diego, CA 92121 > 858-558-3532 > pmcfadden@liai.org > >
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