Hi Lee Check Serotec Inc (servicing USA & Canada) Tel: 1-800-265-7376 , (e-mail: serotec@serotec-inc.com)or visit their website (www.serotec.co.uk). They have canine cell surface markers to B-cells (CD21 like), CD3, CD4, CD5, CD8 and canine CD45 (both purified and RPE conjugated. Another company where you can find canine cell surface marker is VMRD, Inc. in USA (Tel: (800) 222-8673, Web address: www.vmrd.com. Good Luck. Alaa Saad ___________________________________________________________ AstraZeneca R&D Södertälje Safety Assessment, Clinical Pathology, S-151 85 Södertälje, SWEDEN Tel: +46 8 553 258 93 Fax: +46 8 553 288 23 alaa.saad@astrazeneca.com -----Original Message----- From: ltalbot@rdg.boehringer-ingelheim.com [mailto:ltalbot@rdg.boehringer-ingelheim.com] Sent: onsdag, oktober 6, 1999 14:24 To: cyto-inbox Subject: Canine Cytometry Dear All, We are trying to detect cell surface markers to determine B and T cells in canine. Does any one have any suggestions as to who manufacturers antibodies for canine. I see that Pharmingen does, but are they the only one? Plus I am interested in a marker that detects only lymphocytes in canine to reassure myself that I am gating the correct population. CD45 was used in the monkey, and rat, and I recieved the results that I was looking for, but Pharmingen human cell surface molecule CD45 does not cross react with canine. Any suggestions would be greatly appreciated. Thank you in advance. Lee Anne Talbot, Scientist Boehringer Ingelheim Ridgefield CT USA 203-798-4133
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