Lori, This is a common practice. It probably has validity, within the context of a single set of samples, assuming the channel numbers were linear or linear equivalents for a log distribution. Personally, I think calculating the ratio of positive to negative MFI is more useful, as it allows one to normalize among experiments. This, of course, assumes the correct titration, that labeling is saturated, and detection is optimized. MAK. Lori Krueger wrote: > Recently, I was reading an article where the investigators subtracted > the mean fluorescence intensity of the negative (isotype)control > population from the fluorescence intensity of the positively stained > cells. I was wondering what folks thought of this practice. Is this > legitimate? and if so (or not) under what circumstances? Thanks in > Advance. Lori Krueger -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry <http://www.cancer.med.umich.edu/flow_cytometry> phone: 734-647-3216 fax: 734-936-7376 kukuru@umich.edu
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