The obvious interpretation is that they are 'fat' nuclei in G1. Put another
way , they are nculei from cells in G1 but for some reason they are much
biger diameter.
You do not say how the cells were treated prior to analysis or how they
were prepared for analysis. It is possible that either some drug treatment
has caused the nuclei to expand or that this has occurred during
preparation.
You should look at the stained nuclei under a fluorescence microscope to
see if any of them are appreciably larger.
Michael Ormerod
34 Wray Park Road
Reigate RH2 ODE
Telephone: voice & FAX: (0)1737 241726
Mobile telephone: 0802 293242
Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
Message text written by "ray hester"
>
Several times over the last few months when analyzing cells that have been
stained with propidium iodide we have seen, using pulse processing, cells
that have an FL2W signal that would seem to qualify them as doublets and
yet their FL2A is similar to that of a singlet. E.g., in the example
below, if xxx represent G0/G1 single cells, yyy are G2/M single cells, and
zzz doublets, what are the cells represented by ???? ?
yyyy zzzz
FL2A
xxxx ??????????
FL2W
Thanks for any suggestions/answers.<
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