In a message dated 9/28/99 11:20:48 AM, VPLAINETR@niaid.nih.gov writes:
<< Does anyone know companies that supply good blocking anti-CD80 mAbs besides
R&D?
Thank you in advance.
Varatda Plainetr,MD >>
Exalpha has an excellent blocking CD80 mAb. You can reach Exalpha at
800.395.1137, or 617.558.3265, or by fax: at 617.989.0404.
Cheers,
John Castracane
Exalpha
Boston, MA
Info on the antibody follows:
Anti human CD80 (B7-1)
Specificity: Recognizes the extra-cellular domain of human CD80
(also called B-7).
Cell
Distribution: CD80 is rapidly induced on the surface of activated B
cells, T cells and monocytes and is also expressed at high levels on
dendritic cells.
Function: CD80 is one of two ligands (the other CD86) for CTLA4 and
CD28. CD80 acts as co-stimulatory molecule in eliciting T-cell help during
antigen presentation. Antigen presentation in the absence of sufficient
costimulation involving CD86/CD80 can induce tolerance. CD80 appears to play
a role distinct from CD86 in T helper cell differentiation.
Isotype: mIgG1.
Clone: AS29
Concentration: 1 mg/ml
Formulation: Provided as 0.2 mm sterile filtered solution in phosphate
buffered saline with 0.1% sodium azide.
Applications: Neutralization. AS29 has been shown to to block the
interaction of soluble hCD80 with hCTLA-4.
Immunoprecipitation. This antibody has been used to immunoprecipitate
>90% of CD80 in solution using a double antibody method. (Midgley, AR and
Hepburn, MR, Methods Enzymol. 70, 266, 1980)
Immunogen: Recombinant extracellular domain of human CD80.
Does not bind the extracellular domain of CD86.
Antibody Source: In vitro cell culture, proA purified.
Antibody Purity: > 95 % by SDS PAGE
Storage: Antibodies should be stored at 4-8o C. If longer storage
is required, aliquote and store frozen at -20o C. Avoid freeze/thaw cycles.
Ordering
Information Form Vial Size Catalog #
Pure 0.5 mg 0801
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