We deplete monocytes from total peripheral blood mononuclear cells(PBMC)
by by incubating them on polystyrene tissue culture flasks in complete RPMI
medium for 45 min at 37'C, at 2-3x10^6 cells/ml, 12-15 ml/one T75 tissue
culture flask or 100mm tissue culture dish. (Monocytes will stick to
polystyrene
and we harvest the non-adherent cells). You can transfer the non-adherent
cells
to new flasks/dished and deplete them for a further 45 min if you need
complete depletion. Works fine with bovine and mouse PBMC.
Good luck
Sathi
sathy@vasci.umass.edu
>To those working with monocytes,
>Are there any drugs (or cytokines/chemokines) that specifically kill or
>inhibit monocyte development in primary cell cultures but are relatively
>non-toxic to other cell types?
>Many thanks in advance.
>Robert Nordon
>Graduate School of Biomedical Engineering,
>University of NSW.
>Sydney, Australia.
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