Hi Paul This is the (scattergun) panel we use to characterise a new acute leukaemia: CD14/glycophorin A/45, CD34/33/45, CD15/13/45, CD7/11b/HLA-Dr, CD5/10/19, CD61/62/45, CD8/4/3, CD3/56+16/20, CD2/19/45, Kappa/Lambda/19, TdT. We also do myeloperoxidase/lactoferrin if myeloid lineage is established. For followups on known leukaemias we use a panel of antibodies which were found to characterise the disease, all with CD45Cy5, and analyse the blast population on the CD45/SS histogram. Beth Rees Royal Hobart Hospital Hobart Tasmania >How does one pursue an acute leukemia using three color analysis? How do >you know where to look for the blast population, if the blasts are < 10% >of the total? Do you use a >CD45 Vs LSS histogram setup? We are new at this particular aspect and >would appreciate any help available. Also any specific panels that are >run for ALL ,AML,AMML etc. Thanks in advance. > >Reply to: pmk3351@usl.edu <mailto:pmk3351@usl.edu> or the group. > ><!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN"> ><HTML> ><HEAD> > > ><META content='"MSHTML 4.72.3110.7"' name=GENERATOR> ></HEAD> ><BODY bgColor=#ffffff> ><DIV><FONT color=#000000 size=2>How does one pursue an acute leukemia using >three color analysis? How do you know where to look for the blast >population, if >the blasts are < 10% of the total? Do you use a</FONT></DIV> ><DIV><FONT color=#000000 size=2>CD45 Vs LSS histogram setup? We are >new at >this particular aspect and would appreciate any help available. Also any >specific panels that are run for ALL ,AML,AMML etc. Thanks in >advance.</FONT></DIV> ><DIV><FONT color=#000000 size=2></FONT> </DIV> ><DIV><FONT color=#000000 size=2>Reply to: <A >href="mailto:pmk3351@usl.edu">pmk3351@usl.edu</A> or the >group.</FONT></DIV></BODY></HTML>
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