Alessandra Nardin wrote: > > Dear all: > > we are searching for a dead-cell exclusion die to be detected in FL-4 (we > have a 2 laser FACSCalibur). Has any of you had any experience with TO-PRO-3 > (sold by Molecular Probes), so to either suggest or warmly disencourage its > use? Alternatively, did you come across to some other possible candidate? > Thanks. You didn't mention the cell type that you were using, however we've had some good results with this dye with bacteria on an Elite (633ex / 675 em) and on a Microcyte (635ex / >650em). The important things were to keep incubation times short (~1 min) and concentration low (0.1 uM) and of course appropiate controls must be run. If you are using animal cells the timings / concentrations etc may be very different. If you are using plant cells you probably won't get a good topro3 signal because of the autofluorescence. Hope that helps, Haze ---------------------------------------------------------- | Hazel Davey hlr@aber.ac.uk | |Sefydliad y Gwyddorau Biolegol*Inst. Biological Sciences| |Prifysgol Cymru * University of Wales| | ABERYSTWYTH, Ceredigion, CYMRU / WALES SY23 3DD | | http://pcfcij.dbs.aber.ac.uk/index.htm |
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