Freeze whole blood or buffy coats??

From: Ress, S, Stan, Dr (sress@uctgsh1.uct.ac.za)
Date: Fri Aug 27 1999 - 17:42:47 EST 

Hi Folks,

This question has been posted before by myself and others, but I'm 
still in the dark, so here I go again...

Does anyone have a protocol for freezing whole blood that 
would permit subsequent flow cytometry on the thawed sample? We have 
a study starting but the ab have not all arrived.

Alternatively, is there any data/personal observations on comparison 
of frozen buffy coats vs ficoll gradient PBMNC with regard to flow 
cytometry? There must be some information on this, someone must have 
tried it!

Any data or advice would be greatly appreciated.

Thanks,

Stan

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