Is soluble or immobilized CD3mab preferable for lymphocyte activation? I will try to activate lymphocytes by CD3 mab (together with soluble CD28 mab and possibly after antigen stimulation) and measure intracellular cytokines by Flow. In some papers soluble anti-CD3 (conc. 4 microgram/ml) is used, but in most papers immobilized anti CD3 is used. Is there any difference between the two? Unni Haddeland, ph.D. Research Laboratory University College of Akershus Ringstabekkvn 105 1365 Bekkestua NORWAY
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