Can anyone clarify for me the definition of memory and naive lymphocyte populations as detected by CD45RA and CD62L? Our system is lysed whole blood labeling using CD45RA and CD62L in combination with CD4 and CD8 for evaluation of memory and naive cells in HIV infected children. 1] Are all RA+ 62L+ cells naive cells? 2] What are RA+ 62L- cells? I have seen these referred to in the literature as memory cells and also as naive or activated naive [as opposed to RA+ 62L+ which were called resting naive]. Have these cells seen antigen? 3] What are RA- 62L+ cells? My understanding was that 62L was lost prior to the RA to RO conversion. Why would cells which no longer express RA still express 62L? 4] Do memory cells eventually become RA- 62L-? ie the most mature cells are the double negatives? 5] As CD4s and 8s encounter antigen is the sequence of changes in expression of Ra and 62L the same for both subsets? Thanks for any info. Regards, Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] fax: 516-562-2866 8/13/99 1:21:38 PM E-mail: thomasm@nshs.edu --------------------------------------------------------
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