Dear Kathy, The only interference (false hight counts) that we get with TO or DEQTC is from Howell Jolly bodies and RBC parasites. Did you check for that? It would be nice to know if this represents no problem with the sysmex or the advia: does anyone has some experience on that topic? Dirk Prof. Dirk Van Bockstaele, PhD Head Flow Cytometry Facility Laboratory of Hematology Antwerp University Hospital Belgium > ---------- > Van: Altig,Kathy[SMTP:kaltig@swmedctr.com] > Verzonden: donderdag 22 juli 1999 1:16 > Aan: Cytometry Mailing List > Onderwerp: aberrant retic > > > We are in the process of putting retics back into hematology (where they > belong). A Sysmex SE9500/R Alpha II has been installed and we are doing > correlations. The Sysmex uses "auromino" instead of thiazole orange. > Today we performed a retic enumeration on a cancer patient and got very > different answers: > Thiazole orange (FACSCalibur) 5.0% abs = > 191(x1000)/ul > Auromino (Sysmex SE9500/R) 0.8% abs = > 30(x1000)/ul > methylene blue (manual) 0.8% abs = > 30(x1000)/ul > > Initially we reported the FACS result, as usual. Then the physician > called and said he did not believe the result; would we please recheck > it. We repeated the thiazole orange on the FACS, and it repeated > itself. Since the situation of the patient fit with the manual/Auromino > values, we sent out a corrected report using the manual values. Does > anyone have any idea as to why the thiazole orange count was so off the > wall? Could the patient be on some particular drug therapy that would > interfere? We have asked for a new sample on the patient, and a > detailed drug history. > > I would greatly appreciate any shedding of light on this subject. > > Thank you! > Kathy Altig > Southwest Washington Medical Center > Vancouver, WA > kaltig@swmedctr.com > 360-256-2206 >
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