Dirk, Some people took offense by what you said but I thought you were kidding, or as I say "being cute" (I think I pointed that out in my email). We need to not take offense easily. Humor is a great way to get through life without going insane so I hope we can all continue to kid each other. You just got back from holiday? I am green with envy. You guys get such long holidays. Here in the states it is usually a week and back to the grind. You lucky dog! Maryalice >Hi Maryalice (and others engaged in this discussion), >I'm back from holiday and realise that there has been some discussion about >my remark "not long in the business I guess". Off course this was not ment >to be offensive!! I get this type of questions from my students practically >every day and I give them the same type of answer, but off course, then you >can look them in their face so that they see that your smiling while your >tapping on their shoulder. Please keep in mind that e-mail is >international, maybe we have another sense of humor and are easily >misunderstood: after all we have the disadvantage of having to commmunicate >in another language than our own: think of all you US/UK people having to >use french, german or dutch to respond to messages! >And the question was certainly a good one: the evaluation of kappa/lambda on >B-cells is perhaps the most important test in rapid screening for clonal >B-populations in B-NHL patients: it should thus be carried out with the >greatest scrutiny! > >Dirk > >Dirk Van Bockstaele >Laboratory of Hematology >Head Flow Cytometry Unit >Antwerp University Hospital >Belgium >tel. 32 3 821 3900, fax 32 3 825 1148, >e-mail dirk.van.bockstaele@uza.uia.ac.be > > > > >> ---------- >> Van: Maryalice Stetler-Stevenson[SMTP:stetler@box-s.nih.gov] >> Verzonden: vrijdag 23 juli 1999 16:27 >> Aan: Cytometry Mailing List >> Onderwerp: dumb questions >> >> >> I have yet to meet someone who never asks dumb questions. I ask my >> share and I am proud that I am not afraid to ask a dumb question. At least >> I get the needed information when I need it. I don't, however, feel that >> the original CLL question was dumb. CLL is difficult to detect >> Kappa/lambda >> staining. We always use at least 2 different light chain reagents because >> sometimes one will give you an answer and the other won't. Of course we >> wash the bood but we do a whole blood lysis and we don't incubate the >> cells >> (we studied this and didn't find it necessary- Cytometry (Communications >> in >> Clinical Cytometry)26:243-252,1996). However sometimes, no matter what you >> do, the light chain staining is so very dim that it is really hard to >> detect. I think the discussion on this point was good and I hope all will >> feel free to join me in asking dumb questions. We will all learn in the >> process. >> I like to think that Dirk Van Bockstaele was just kidding and being >> cute in his reply "Not long in the business I guess? " and not intending >> to >> insult. Let's cut him a break too. >> >> Maryalice >> >> >> I'm not offended by all the dumb blonde jokes because I know I'm not >> dumb... and I also know that I'm not blonde. -Dolly Parton- >> Maryalice Stetler-Stevenson >> Director Flow Cytometry Unit >> Laboratory of Pathology, NCI, NIH >> Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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