Hi David, There have been publications correlating coulter volume and FSC measurements in the "early" days: I recall papers by Mullaney's group back in 1969! You should try to get the first edition of "flow cytometry and sorting" edited by Melamed, Mullaney and Mendelsohn (1979) where you can find a wealth of "old" references. Dirk Prof. Dirk Van Bockstaele, PhD Head Flow Cytometry Facility Laboratory of Hematology Antwerp University Hospital Belgium > ---------- > Van: David Lloyd[SMTP:lloyddr@novell2.bham.ac.uk] > Antwoord naar: D.R.Lloyd@bham.ac.uk > Verzonden: dinsdag 3 augustus 1999 14:58 > Aan: Cytometry Mailing List > Onderwerp: Very basic question > > > Dear all > > Please excuse this very basic question, I would be very grateful for any > wisdom you can give. > > It is generally accepted that "forward scatter = size" within certain > limits; > shape, refractive index etc. > > Does this knowledge come solely from the theoretical physics/optical > properties > of particles in laser beams, or is there any empirical evidence > correlating fs > with coulter volume (or any other size measurement)? I have looked for > such > evidence but have not been able to find any; perhaps it is further back in > > history than I can search. > > If anyone knows of any published empirical evidence I would be very > grateful if > you could share it with me. > > Thanks in advance > > > > David R Lloyd > University of Birmingham > England > > t; +44 (0)121 414 5264/7 > e; D.R.Lloyd@bham.ac.uk > http://www.bham.ac.uk/chemeng/actg/actghome >
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