Can someone tell me what are the practical differences, if any, between the DNA dyes Hoechst 33342, Hoechst 33258 and Hoechst 2495? Do they have different binding properties? Different retention properties? We are trying to label sperm in vivo in snails. When we use Hoechst 33342 the cells look great while in the male, but once transfered to the female the label seems to leak out. Thanks, Ronald Chase, Dept. Biology, McGill Univ. 1205 Av. Docteur Penfield Montreal, Quebec, H3A 1B1, Canada TEL 514-398-6422 FAX 514-398-5069 http://www.mcgill.ca/Biology/faculty/chase/
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