Hi! First of all, I'd like to thank all who have responded to my email about rabbit monocyte marker. It was well appreciated! I am having trouble to differentiate rabbit heterophils from monocytes in the light scatter plot of a hematology counter. The heterophils are as large as the monocyte, and the nucleus can be tight and compact, similar to that of a monocyte. They differ in that heterophils have small granules in the cytoplasm. So, I usually obtain a population of cells with higher granularity in the mononuclear gate of a 90o/10o plot. As a result, heterophils are counted as monocytes and I get innacurate percentages of the cell populations. Does anybody have experience with rabbit cells? How could I differentiate the two populations without overlap? Thanks!
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