Re: BrdU Incorporation

From: Simon Monard (smonard@trudeauinstitute.org)
Date: Thu Jul 29 1999 - 08:55:59 EST

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Hi 

I am confused

 You wrote: "I used Pharmigen's anti-BrdU, Biotin-mIgG1 as secodary antibody, and SA-APC
or SA-perCP. "

Biotin -mouse IgG1 as secondary antibody? Biotin anti mouse IgG1 I assume. Why are you using three steps?

 In my experience the BD anti BRDU is the best.

Simon Monard
Trudeau Institute
NY

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