Dear Group; I am looking for BrdU incoporation protocols other than Becton Dickinson's protocol. I have tried twice with B.D.'s protocol but it didn't work for my Hela cells. Because my Hela cells express GFP, I can't use direct labeling. I used Pharmigen's anti-BrdU, Biotin-mIgG1 as secodary antibody, and SA-APC or SA-perCP. Thanks for any suggestions. Bill Throndset Flow Cytometry Consultant Rigel, Inc. 240 East Grand Avenue South San Francisco, CA 94080 650-624-1231 650-624-1101 (fax) bthrondset@rigel.com
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:48 EST