Richard McFarland PhD, MD wrote: > > Flowers- > > I agree with all of Maryalice's astute comments and would like to add one > comment of my own to the discussion. > > When we are evaluating B-cell malignancies we routinely run the following > two four color tubes (in addition to others not directly pertinent to this > discussion) > > mK/mL/CD5/CD19; and > pL/pK/CD20/CD38. > > We find as Maryalice suggests that the different sets of light reagents > can allow you to make a diagnosis when one set of reagents might not. We > use one monoclonal set and one polyclonal set and we switch the > fluorochrome- light chain combinations between the two tubes becasue we > find that the PE reagents tend to pull the light chain positve cells away > from the 45 degree line of non-specificity slightly better than the FITC > reagents. Also as Maryalice points out CLL's have characteristically dim > surface immunoglobulin staining. The other common small, mature B lineage > CD5+ malignancy is mantle cell which characteristically has bright > immunoglobulin staining. This distinction is not adequate alone to make the > diffirential diagnosis, but it is helpful. > > Richard McFarland > Instructor > Division of Hematopathology and Immunology > Department of Pathology > UT Southwestern Medical Center at Dallas > Dallas, Texas Hello Fellow Flowers, Who said that it was a dumb qwestion? We should have more of this type (of dumb qwestions!), why? See how many useful tips have come out of it! The novice always learns from the experts out there! -- Warmest regards from: -------------------- Fareed Al-Gurg, Haematology, Dubai Hospital, Dubai - U.A.E. Tel: (09714) 714444 / 7075447 / 512173 Fax: (09714) 441143 / 512121
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