Dear Susan, In my experience with ModFit, Apoptosis modeling requires the analyzer to move the histogram markers. Modfit will always place the apoptosis marker (APOP) over the first major peak which is mostly the (G0/G1) peak. If you move all of your markers over the appropriate peaks, you can then fit the curve. Look at the chi-square value. If the chi-square is in range (0.9 - 3.0), you have a good fit and the data can be trusted. If you still have trouble with the fit, I'm sure their technical support can help you. I agree, the manual isn't that friendly. I've contacted Verity House directly through e-mail with this same question. The response time was short and I was happy with technical support. Email: Verity@vsh.com Also, Don Herbert, Techinical Support, was very helpful with my other questions regarding Sync Wizard. Good Luck, Lora Barsky Children's Hospital Los Angeles Research Immunology/BMT > -----Original Message----- > From: szunino@biologie.uni-erlangen.de > [SMTP:szunino@biologie.uni-erlangen.de] > Sent: Wednesday, July 21, 1999 5:04 AM > To: Cytometry Mailing List > Subject: > > > I would like to get some information from anyone who is successfully using > the Modfit DNA Analysis program for analysis of DNA content, cell cycle, > and especially apoptotic populations. We are having quite a bit of > difficulty getting useful information from this program regarding > apoptotic > populations. We are using mostly PI staining of nuclei for analysis. We > are also using cell lines, not clinical samples, so there is limited > involvement of an aneuploid population. The main trouble is setting the > markers so the data makes sense. We either see an apoptotic population > under the G1 and S phases of the histogram or the apoptotic population is > labeled as debris. We have no consistency in our data with regard to > apoptosis. The manual is not helpful. If anyone out there can help, it > sure would be appreciated. Best regards, Susan Zunino > > Dr. Susan J. Zunino > Lehrstuhl fur Genetik > University of Erlangen > Staudtstr. 5 > 91058 Erlangen > Germany > Tele. 49 9131 8528784 > FAX 49 9131 8528526 > e-mail: szunino@biologie.uni-erlangen.de >
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