Greetings! I am trying to stain for intracellular IFN-gamma and IL-4 in rhesus. Does anyone have any suggestions as to concentration of PMA and ionomycin which work as activators? This activation step is not done in flasks, but is activation of 100 ul whole blood (plus 100 ul of tissue culture media therfore a total volume of 200 ul). I tried to activate 24 hours and got absolutely nothing! (These are however, normal cells, and I only used IFN -gamma this time as the IL-4 is normally lower....or so I was told)
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:42 EST