Hi Jim On the MoFlo, I use a 655LP first to knock down the 647 laser , followed by a 718SP dichroic, passing APC through a 670BP20, and the APC-Cy7 through a 740LP (although a 780BP should work also). Peter Lopez > ---------- > From: Houston, Jim [SMTP:Jim.Houston@stjude.org] > Sent: Thursday, July 08, 1999 1:29 PM > To: Cytometry Mailing List > Subject: Filter selection for Spectrum laser at 647nm mode. > > > To All: > > I want to use the 647 nm line for APC and APC-Cy7 work. > Which filters are reccomended for this arrangement? > What for FL-4 and Fl-5? > > Thanks > > Jim Houston >
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