Re: Chicken RBC Lysis

From: Nigel_LLEWELLYN_SMITH/Europe@europe.bd.com
Date: Sat Jul 10 1999 - 19:20:00 EST


Hi Carol,

Coming from a position of complete ignorance and inexperience of chicken
red cells:

Lysing with conventional agents will be problematic, as these are nucleated
cells.

However, if you had an anti-chicken blood group antibody (if there is such
a thing!) or other red cell surface antigen, that bound complement
(analagous to human IgG1/IgM) then this might let you pick off the red
cells selectively.

Good luck!

Nigel



This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:42 EST