RE: Requestion on Hoechst and Pyronin odd peak

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Another solution would be to suspect some quenching going on. Energy transfere
between the blue emitting hoechst and the blue/green excited Pyronin, but I
would not have thought they are that close enough and the spectra do not match
that well.  Do you perhaps get a signal processing artefarct?

Regards
Gerhard

-----Original Message-----
From:	Plett, Paul A. [SMTP:pplett@iupui.edu]
Sent:	Thursday, July 08, 1999 5:44 AM
To:	Cytometry Mailing List
Subject:	Requestion on Hoechst and Pyronin odd peak


	Thanks fro the replies. The two suggestions relating to a sub G0G1
peak with hoechst and Pyronin staining were the MDR and the p-gp pumps that
may be pumping out the Hoechst.
	This brings up more questions however, that I would like to throw
out into flowland (flow-country sounds more serene :)

	1. We use Verapamil in all the staining steps and also while running
the samples.
	2. We do not see the sub G0/G1 when we stain with hoechst alone,
only when double staining with hoechst and pyronin.

	Any ideas?

	thanks
	artur

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