Requestion on Hoechst and Pyronin odd peak

From: Plett, Paul A. (pplett@iupui.edu)
Date: Wed Jul 07 1999 - 23:43:58 EST 

	Thanks fro the replies. The two suggestions relating to a sub G0G1
peak with hoechst and Pyronin staining were the MDR and the p-gp pumps that
may be pumping out the Hoechst.
	This brings up more questions however, that I would like to throw
out into flowland (flow-country sounds more serene :)

	1. We use Verapamil in all the staining steps and also while running
the samples.
	2. We do not see the sub G0/G1 when we stain with hoechst alone,
only when double staining with hoechst and pyronin.

	Any ideas?

	thanks
	artur

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