Hi Mike, 1. First check in Acquisition & Storage window under Parameters Saved if you are saving FL-3 Area. It could be as simple as that. 2. You can switch P5 and P6 to be from 1-st laser, there should be a pop-up menu there in Det/Amp window under Laser. 3. If both cards (17 and 18) are turned ON in BDPAC/Control Panel, check from Pulse Processing window in CellQuest which card is G1 and which is G2 card by selecting parameter from pop-up window and looking on a card itself to see which red diode is going ON. On my Vantage G1 is Card 18. After that, when you select FL-3 Area from Pop-up in Det/Amp be careful to choose FL-3 Area from a card (pop-up menu is divide on G1 and G2 fields) that has FL-3 for processing. Hope this helps, Sasha On Wed, 30 Jun 1999 21:07:42 EDT MSchwa3722@aol.com wrote: > Could anyone help me out with instrument setttings for the Vantage using > Pheonix flow's APO-BRDU apoptosis kit. My Vantage has its pulse processing > card in slot 17 and I set up my parameters for acquisition with FL-3 area on > parameter 8. I did this because parameters 5 and 6 were apparently set to > use the 2nd laser and I was using only the 488nm Argon laser. After > acquiring the data, which looked fine during acquisition, I wasn't able to > pull up the FL3 Area parameter on my analysis dot plots. If anyone has had > any experience using Pheonix Flow's APO BrdU kit on the Vantage and can help > me out, I'd be much obliged. I spent quite some time preparing these sample > and it would be a shame if I couldn't analyze the data. > > Thanks in Advance. Mike -@CHOC ---------------------- Dr Sasha Sreckovic Dept Path & Micro University of Bristol University Walk Bristol, BS8 1TD, UK Sasa.Sreckovic@bristol.ac.uk 0117-928-8606
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