Hello, everyone, Use of CD4-PE stained PBL's as a biological calibrator was described by Hultin L.E. et al., Medline UI: 98444856. I am just curious if anyone checked stability of PBL's, which were first stained with CD4-PE and THEN cryopreserved in liquid nitrogen. In our hands viability of thawed PBMC's is close to 100%. If M.F.I./CV of CD4+ cells did not change significantly after thawing, then it might be possible to keep a large stock of "calibrating" cells, which were prepared under same conditions, e.g., blood donor(s), Mab affinity, PE/IgG ratio, etc. Also, time should be going much slower in liquid nitrogen, as for other "stabilized" calibrating cells (freeze-dried, formaldehyde treated etc.). Those labs, which have continuous supply of liquid nitrogen, might benefit from this. If this idea doesn't sound entirely stupid to you I'll be happy to hear your comments. Best regards, Yuri YKudinov@CHW.edu -------------------------------- Yuriy Kudinov, postdoctoral fellow Immunotherapy Laboratory St. Vincent Medical Center 201 South Alvarado Street Prof.Office Bldg, Suite 312 Los Angeles, CA 90057 U.S.A.
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