Hello again, My problem this week: 1. how does one define a representative region to study monocytes and granulocytes? 2. how does one calculate the purity of such a region? what we've been doing so far, is the same thing we do for lymphs, namely, define R1 on the ssc/fsc and using it "highlight" corresponding populations on the CD45/14 plot, then draw R2 on the CD45/14 plot and use it as a gate for a third plot plot (CD45/14) on which the LR quadrant is our purity. This doesn't quite work for monos/grans. The ssc/fsc plot resolves the two populations quiet well, but CD45/14 has an overlap between the two. I wonder if anyone here has worked with these populations and would like to contribute their thoughts? Specifically, we are setting this template up for a BPD and sepsis study where we look at 18/11b, 18/62L, and 15/11b. thanks for any input! === `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3952 | `---------------------------------------------` _________________________________________________________ Do You Yahoo!? Get your free @yahoo.com address at http://mail.yahoo.com
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