Dear summer flowers, We have for some time cursed a phenomenon which we have referred to as the "pölsa". The phenomenon occurs in isotype controls at various frequencies depending on the type of material analysed, and leads to the formation of a tail that stretches across the dotplot window. The problem seems to be most frequent when analyzing chimeric NOD/SCID mice transplanted with human bone marrow cells. Does anyone have any solution to this or thoughts around how this should be interpreted or possible be dealt with? Carl-Magnus Högerkorp Stem cell laboratory Department of Internal Medicine University Hospital of Lund Sweden
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