--- On Wed, 30 Jun 1999 15:53:17 -0400 msiegel@qlab.quintiles.com wrote: > >I would like to know if anybody has had any problems with low normal ranges for >CD-8 using the Epics XL and Coulter antibodies. We have only run forty normals >and our ranges is 6.45 - 39.95. Are there any suggestions on why this might >be so low? Here are som e consideratons: Is this the actual range or the calculated normal range? How was it calculated: median with 10th and 90th %, mean +\- 2SD, etc.? Are these values for all CD8+ or CD3CD8? What selection criteria was used for the donors? What criteria was used to judge the data: minimum lymphocyte purity and recovery, check lymphosum, etc.? The literature lists the followint for adult CD8 ranges [calculated by different methods]: 19-48% Clin Immunol Immunopathol 60: 190 1991 20-43% Acta Pediatr 81: 125 1992 14-46% Clin Exp Immunol 88: 243 1992 31-40% J Peds 120: 216 1992 17-42% Clin Immunol Immunopathol 84: 46 1997 I also think the CD8 F McAb has been implicated in the escapee phenomenon. You may want to check your data for this. Routinely checkingn lymphosum and tube to tube variation is a good way to detect this. Good luck, Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] fax: 516-562-2866 7/6/99 11:51:44 AM E-mail: thomasm@nshs.edu --------------------------------------------------------
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:41 EST