> > >Content-Type: message/rfc822 >Content-Disposition: inline > >Return-path: MSchwa3722@aol.com >From: MSchwa3722@aol.com >Full-name: MSchwa3722 >Message-ID: <482520dd.24ac195e@aol.com> >Date: Wed, 30 Jun 1999 21:07:42 EDT >Subject: Vantage Inst Settings >To: cyto-inbox >MIME-Version: 1.0 >Content-Type: text/plain; charset="us-ascii" >Content-Transfer-Encoding: 7bit >X-Mailer: AOL 4.0 for Windows 95 sub 4 > >Could anyone help me out with instrument setttings for the Vantage using >Pheonix flow's APO-BRDU apoptosis kit. My Vantage has its pulse processing >card in slot 17 and I set up my parameters for acquisition with FL-3 area on >parameter 8. I did this because parameters 5 and 6 were apparently set to >use the 2nd laser and I was using only the 488nm Argon laser. After >acquiring the data, which looked fine during acquisition, I wasn't able to >pull up the FL3 Area parameter on my analysis dot plots. If anyone has had >any experience using Pheonix Flow's APO BrdU kit on the Vantage and can help >me out, I'd be much obliged. I spent quite some time preparing these sample >and it would be a shame if I couldn't analyze the data. > >Thanks in Advance. Mike -@CHOC Hi Mike, Right, the FL3 area is measured in slot 17, however if the Vantage is not "configured" for the pulse processing board you will not get a signal. So to check: Exit cellquest; go to control panels in system folder; click on BDMAC, click on configuration and see if there's a tick in Pulse board 1 or 2; you need 2. You can click on both boards as you can use the one board in both slots depending on what you are measuring. Restart computer. If this is not the source of the problem; go to pulse processing under cytometer in CellQuest and set the G2 card to SSC and Fl3 as this is what's measured here with single laser or, instead of SSC use FL3 twice. ORrrrr you might have to play with the dead time (board in slot nr 13). Have no experience with the kit. Good Luck Ann EMBL Heidelberg
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