Hi Martha, We use to have problems with CD8 counts due to FITC labeled mAbs. In comparison to PE labeled mAbs we always had at least 10% lower counts with FITC labeled antibodies. However we never found a wide range for healthy donos. Ours results were always between 25-32%. I am sorry if I did not help. Good Luck, Olindo ---------- De: msiegel@qlab.quintiles.com[SMTP:msiegel@qlab.quintiles.com] Enviada: Quarta-feira, 30 de Junho de 1999 16:53 Para: Cytometry Mailing List Assunto: Range for normal CD-8 I would like to know if anybody has had any problems with low normal ranges for CD-8 using the Epics XL and Coulter antibodies. We have only run forty normals and our ranges is 6.45 - 39.95. Are there any suggestions on why this might be so low? Martha Siegel Quintiles Laboratories, Ltd. 5500 Highlands Parkway, Suite #600 Smyrna, Georgia 30082 770-434-8492 FAX: 770-434-7255
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